CRL-2021 MEG-01 人成巨核細(xì)胞白血病細(xì)胞

ATCC® Number: CRL-2021™

Designations: MEG-01

Depositors: M Tekeuchi, MJ Fitzgerald

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Homo sapiens （human）

Morphology: lymphoblast

CRL-2021 MEG-01 人成巨核細(xì)胞白血病細(xì)胞

Source: Disease: chronic myelogenous leukemia （CML）

Cell Type: megakaryoblast;

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CRL-2021 MEG-01

Applications: transfection host （Nucleofection technology from Lonza）

Antigen Expression: CD41 +; CD61 +; CDw14 +

DNA Profile （STR）: Amelogenin: X,Y

CSF1PO: 10

D13S317: 8

D16S539: 9

D5S818: 13

D7S820: 11

THO1: 7

TPOX: 8,11

vWA: 16

Cytogenetic Analysis: hyperdiploid; modal number = 56 to 58; the Philadelphia chromosome （Ph1） is present

Age: 55 years

Gender: male

Comments: The MEG-01 cell line was derived in 1983 at the Nagoya University School of Medicine, Nagoya, Japan from bone marrow cells taken from a patient in megakaryoblastic crisis of CML.

The cells are positive for cytoplasmic Factor VIII and surface GPIIb/IIIa, periodic acid - Schiff （PAS） reaction, alpha naphthyl acetate esterase and acid phosphatase.

They are negative for myeloperoxidase, alpha naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase and alkaline phosphatase.

The stain positively with monoclonal antibodies BA-1 （anti B cell, granulocyte）, HPL-3 （anti gpIIb/IIIa） and 20.3 （anti monocyte, plaet）.

They are negative for other lymphoid and myeloid seris antigens.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Subcultures are prepared by scraping the adherent cells into the medium. From the resulting suspension dilute cells to a concentration 1-2 X 10exp5 cells/ml into fresh medium in a new flasks. Keep culture below approximate density of 10exp6 cells/ml.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Subcultures are prepared by scraping the adherent cells into the medium, and diluting the resulting suspension into fresh medium in new flasks.

Doubling Time: 36 to 48 hrs

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001

recommended serum:ATCC 30-2020

References: 23348: Ogura M, et al. Establishment of a novel human megakaryoblastic leukemia cell line, MEG- 01, with positive Philadelphia chromosome. Blood 66: 1384-1392, 1985. PubMed: 2998511

CRL-2021 MEG-01