CRL-2632 Pfeiffer 人彌漫性大細胞淋巴瘤B淋巴細胞

ATCC® Number: CRL-2632™ Price: $379.00

Designations: Pfeiffer

Depositors: H Ben-Bassat

Isotype: IgG kappa

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens （human）

Morphology: lymphoblast

Source: Disease: diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma

Derived from metastatic site: pleural effusion

Cell Type: lymphoblast B lymphocyte;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

CRL-2632 Pfeiffer 人彌漫性大細胞淋巴瘤B淋巴細胞

Isolation: Isolation date: 1992

Antigen Expression: CD10; Homo sapiens

CD19; Homo sapiens

CD20; Homo sapiens

CD38; Homo sapiens

CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289]

DNA Profile （STR）: CSF1PO: 10, 11

D13S317: 11, 12

D16S539: 12

D5S818: 10, 13

D7S820: 8, 12

THO1: 9, 9.3

TPOX: 9

vWA: 17, 18

Amelogenin: XY

Cytogenetic Analysis: The cell line contains several chromosomal abberations including the typical t（14;18）（q32;q21） translocation of follicular lymphomas.

Age: adult adult

Gender: male

Ethnicity: White

Comments: The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma （DLCL） with cleaved and non-cleaved nuclei. [53289]

The cells are negative for Epstein-Barr virus. [53289]

This cell line is a model system for studying non-Hodgkin lymphomas.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days （depending on cell density）

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Doubling Time: 24 to 30 hrs

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001

recommended serum:ATCC 30-2020

References: 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273

ATCC® Number: CRL-2632™ Price: $379.00

Designations: Pfeiffer

Depositors: H Ben-Bassat

Isotype: IgG kappa

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens （human）

Morphology: lymphoblast

Source: Disease: diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma

Derived from metastatic site: pleural effusion

Cell Type: lymphoblast B lymphocyte;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1992

Antigen Expression: CD10; Homo sapiens

CD19; Homo sapiens

CD20; Homo sapiens

CD38; Homo sapiens

CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289]

DNA Profile （STR）: CSF1PO: 10, 11

D13S317: 11, 12

D16S539: 12

D5S818: 10, 13

D7S820: 8, 12

THO1: 9, 9.3

TPOX: 9

vWA: 17, 18

Amelogenin: XY

Cytogenetic Analysis: The cell line contains several chromosomal abberations including the typical t（14;18）（q32;q21） translocation of follicular lymphomas.

Age: adult adult

Gender: male

Ethnicity: White

Comments: The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma （DLCL） with cleaved and non-cleaved nuclei. [53289]

The cells are negative for Epstein-Barr virus. [53289]

This cell line is a model system for studying non-Hodgkin lymphomas.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days （depending on cell density）

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Doubling Time: 24 to 30 hrs

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001

recommended serum:ATCC 30-2020

References: 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273

CRL-2632 Pfeiffer 細胞