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簡要描述：CRL-2632 Pfeiffer 人彌漫性大細胞淋巴瘤B淋巴細胞，ATCC 細胞|細胞系|細胞株|細胞,細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件
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CRL-2632 Pfeiffer 人彌漫性大細胞淋巴瘤B淋巴細胞
ATCC® Number: CRL-2632™ Price: $379.00
Designations: Pfeiffer
Depositors: H Ben-Bassat
Isotype: IgG kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens （human）
Morphology: lymphoblast
Source: Disease: diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma
Derived from metastatic site: pleural effusion
Cell Type: lymphoblast B lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-2632 Pfeiffer 人彌漫性大細胞淋巴瘤B淋巴細胞
Isolation: Isolation date: 1992
Antigen Expression: CD10; Homo sapiens
CD19; Homo sapiens
CD20; Homo sapiens
CD38; Homo sapiens
CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289]
DNA Profile （STR）: CSF1PO: 10, 11
D13S317: 11, 12
D16S539: 12
D5S818: 10, 13
D7S820: 8, 12
THO1: 9, 9.3
TPOX: 9
vWA: 17, 18
Amelogenin: XY
Cytogenetic Analysis: The cell line contains several chromosomal abberations including the typical t（14;18）（q32;q21） translocation of follicular lymphomas.
Age: adult adult
Gender: male
Ethnicity: White
Comments: The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma （DLCL） with cleaved and non-cleaved nuclei. [53289]
The cells are negative for Epstein-Barr virus. [53289]
This cell line is a model system for studying non-Hodgkin lymphomas.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days （depending on cell density）
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Doubling Time: 24 to 30 hrs
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001
recommended serum:ATCC 30-2020
References: 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
ATCC® Number: CRL-2632™ Price: $379.00
Designations: Pfeiffer
Depositors: H Ben-Bassat
Isotype: IgG kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens （human）
Morphology: lymphoblast
Source: Disease: diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma
Derived from metastatic site: pleural effusion
Cell Type: lymphoblast B lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1992
Antigen Expression: CD10; Homo sapiens
CD19; Homo sapiens
CD20; Homo sapiens
CD38; Homo sapiens
CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289]
DNA Profile （STR）: CSF1PO: 10, 11
D13S317: 11, 12
D16S539: 12
D5S818: 10, 13
D7S820: 8, 12
THO1: 9, 9.3
TPOX: 9
vWA: 17, 18
Amelogenin: XY
Cytogenetic Analysis: The cell line contains several chromosomal abberations including the typical t（14;18）（q32;q21） translocation of follicular lymphomas.
Age: adult adult
Gender: male
Ethnicity: White
Comments: The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma （DLCL） with cleaved and non-cleaved nuclei. [53289]
The cells are negative for Epstein-Barr virus. [53289]
This cell line is a model system for studying non-Hodgkin lymphomas.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days （depending on cell density）
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Doubling Time: 24 to 30 hrs
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001
recommended serum:ATCC 30-2020
References: 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
CRL-2632 Pfeiffer 細胞
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