CRL-2279 MS1 小鼠胰島內皮細胞 的詳細介紹

CRL-2279 MS1 小鼠胰島內皮細胞

ATCC® Number:  CRL-2279™

Designations:  MS1 （MILE SVEN 1）

Depositors:   JL Arbiser

Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]

Shipped:  froz

Organism: Mus musculus （mouse）en

Medium & Serum:  See Propagation

Growth Properties: adherent

Morphology: endothelial

CRL-2279

Source: Organ: pancreas

Strain: C57BL/6

Tissue: islet of Langerhans; endothelium

Cell Type: SV40 transformed

Cellular Products: tissue inhibitor of bioreactive matrix metalloproteinase （high levels）

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Receptors: vascular endothelial growth factor （VEGF）, expressed

Comments: MS1 is a pancreatic islet endothelial cell line established in 1994.

Primary islet endothelial cells were transduced with a temperature sensitive SV40 large T antigen （tsA-58-3） constructed and screened for resistance to G418.

Resistant colonies were isolated in cloning rings and screened for uptake of diI-Ac-LDL.

The line retains many properties of endothelial cells including uptake of acetylated LDL and expression of both Factor VIII related antigen and VEGF receptor.

The cells are useful for the study of signal transduction of angigenic factors.

It is the only line known that can give rise to benign hemangiomas.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.

Atmosphere: air, 95%; carbon dioxide （CO2）, 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22603: Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347

51452: Arbiser JL, et al. Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR-1 and VEGFR-2 in vivo. Am. J. Pathol. 156: 1469-1476, 2000. PubMed: 10751370