CCL-219 L1210 小鼠白血病細胞

ATCC® Number:  CCL-219™

Designations:  L1210

Depositors:   EM Jensen

Biosafety Level: 1

Shipp:  frozen

Medium & Serum:  See Propagation

Growth Predoperties: suspension

Organism: Mus musculus （mouse）

Morphology: lymphoblast

CCL-219 L1210 小鼠白血病細胞

Source: Disease: lymphocytic leukemia

Strain: DBA subline 212

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Tumorigenic: Yes

Reverse Transcript: positive

Cytogenetic Analysis: modal number = 40; range = 32 to 80.

Mouse karyotype with a diploid stemline number of 40 chromosomes.

Age:  8 months

Gender:  female

Comments: Tested and found negative for ectromelia virus （mousepox）.

The line was established from a tumor in female #234 that developed following skin paintings with 0.2% methylcholanthrene in ether. [22513]

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 viable cells/ml.

Medium Renewal: Add fresh medium （20% to 30% by volume） every 2 to 4 days

Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time:  8 to 10 hrs

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002

recommended serum:ATCC 30-2040

References: 22513: . . J. Natl. Cancer Inst. 10: 179-192, 1949.

26055: . . J. Natl. Cancer Inst. 36: 405-421, 1966.

26056: . . Cancer Chemother. Rep. 51: 451-453, 1967.

32923: Young SW, et al. Gadolinium（III） texaphyrin: a tumor selective radiation sensitizer that is detectable by MRI. Proc. Natl. Acad. Sci. USA 93: 6610-6615, 1996. PubMed: 8692865

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