CRL-1420  MIA PaCa-2 人胰腺癌細胞 的介紹

ATCC® Number: CRL-1420™ Price: $279.00

Designations: MIA PaCa-2

Depositors: A Yunis

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent, single cells and loosely attached clusters

Organism: Homo sapiens （human）

Morphology: attached epithelial with floating rounded cells

Source: Organ: pancreas

Disease: carcinoma

Cellular Products: human colony stimulating factor, subclass I （CSF-I）; plasminogen activator

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Isolation: Isolation date: 1975

DNA Profile （STR）: Amelogenin: X

CSF1PO: 10

D13S317: 12,13

D16S539: 10,13

D5S818: 12,13

D7S820: 12,13

THO1: 9,10

TPOX: 9

vWA: 15

Cytogenetic Analysis: This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent.

Isoenzymes: G6PD, B

Age: 65 years

Gender: male

Ethnicity: Caucasian

Comments: The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. [58034]

The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximay 19%. [58034]

Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207

References: 1057: Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590

22291: Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line （MIA PaCa-2）. J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740

49623: Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455

CRL-1420  MIA PaCa-2 人胰腺癌細胞